組織について／魚病研究センター (Research Center for Fish Diseases)

病原体グループ (Etiology Group)

　病原体グループは、病原体の特性及び発症要因の解明とそれを利用した防除対策の設計に関する研究開発を行っています。具体的には、水産防疫上の重要疾病であるレッドマウス病、冷水病等の病原体の特性とそれら病原体の感染から疾病発症に至る要因について調べるとともに、それを利用した防除法の開発を行っています。

研究メンバー

グループ長 栗田　　潤
　　　研究員 山﨑　雅俊
　　　研究員 中川　徹優

研究について



レッドマウス病国内分離株で浸漬攻撃後のシロサケ死亡魚　口腔内に発赤が認められる。
 
レッドマウス病原因菌の培地上のコロニー
 
伝染性造血器壊死症ウイルス（IHNV）実験感染ニジマス死亡魚　眼球突出が認められる。
 
魚体へのエレクトロポレーションによるIHN DNAワクチン接種。


魚介類のための病理組織学の方法　(Sampling Methods of Histopathology for Fish and Shellfish)

日本語版　　　　　English

研究課題

  • 病原体特性及び発症要因の解明とそれを利用した防除技術の開発(2016-2020:交付金)
  • ブリ類の微胞子虫症ワクチンの開発に向けた免疫システムの解明（2018-2019: 科学研究費助成事業　挑戦的研究（萌芽））
  • 冷水病ワクチンの開発に有用な菌体表材タンパク質の探索（2019:交付金（シーズ研究））
  • マダイイリドウイルス病等の重要疾病に対するワクチンの開発（2018-2022:農林水産技術会議　戦略的プロジェクト研究推進事業）
  • ギンザケの赤血球封入体症候群ワクチンの改良および迅速な有効性判定技術の開発（2018-2019:交付金プロジェクト）
  • 病原体が不明な水産動物疾病の診断法と防除法の開発（2019-2023:農林水産技術会議　戦略的プロジェクト研究推進事業）
  • タイプIII分泌機構に着眼した魚類エドワジエラ症原因細菌の病原機構の解明（2017-2019: 科学研究費助成事業　研究基盤C）
  • 水産動物疾病のリスク評価及び天然水域における疾病の清浄性確認のための調査（2019: 水産防疫対策委託事業）
  • 水産動物疾病の診断・予防・まん延防止に係る技術開発等及び魚病診断機関の検査精度管理体制の確立（2019: 水産防疫対策委託事業）

発表論文

  • 鈴木 究真・泉 庄太郎・熊川 真二・坂井 貴光・中易 千早. 4種のアユ病原細菌を同時に検出するマルチプレックスPCR法. 日本水産学会誌. 2019. 85(3):340-342.
  • Matsuyama T, Fukuda Y, Takano T, Sakai T, Nakayasu C. Antibody-mediated bacterial killing of Ichthyobacterium seriolicida in Japanese amberjack. Vet Immunol Immunopathol. 2018. 203:73-77.
  • Matsuyama T, Matsuura Y, Inada M, Takano T, Sakai T, Terashima S, Yasuiike M, Fujiwara A, Nakamura Y, Tsuchihashi Y, Odawara K, Iwanaga S, Masaoka T. An Epidemiological Study of Akoya Oyster Disease Using Polymerase Chain Reaction Targeting Spirochaetes Genes. Fish Pathol. 2018. 53 (2): 63-70.
  • Matsuyama T, Sano N, Takano T, Sakai T, Yasuike M, Fujiwara A, Kawato Y, Kurita J, Yoshida K, Shimada Y, Nakayasu C. Antibody profiling using a recombinant protein-based multiplex ELISA array accelerates recombinant vaccine development: Case study on red sea bream iridovirus as a reverse vaccinology model. Vaccine. 2018. 36(19):2643-2649.
  • Sakai T, Kuwada T, Muto Y, Takano T, Yuasa K, Oseko N. Comparative Proteomic Analysis Between Virulent and Less Virulent Strains of Edwardsiella ictaluri Isolated from Ayu Plecoglossus altivelis. 2018. 53(1): 29-35.
  • Neglected viral diseases in freshwater fish farming (O. Haenen, L. Bigarré, Takafumi Ito, J.-C. Avarre, N. Vendramin, 2018, Bulletin of the European Association of Fish Pathologists, 38, 105-114)
  • Virulence marker candidates in N-protein of viral haemorrhagic septicaemia virus (VHSV): virulence variability within VHSV Ib clones (Takafumi Ito, Jun Kurita, Koh-ichiro Mori, Helle Frank Skall, Niels Lorenzen, Niccolò Vendramin, Nikolaj Gedsted Andersen, Katja Einer-Jensen, Niels Jørgen Olesen, 2018, Diseases of Aquatic Organisms, 128, 51-62)
  • Yamasaki M, Sakai T, Ito T, Mori K. Bactericidal Effects of Disinfectants on Yersinia ruckeri. Fish Pathol. 2017. 52(4): 198-201.
  • Takano T, Nakamura Y, Matsuyama T, Sakai T, Shigenobu Y, Sugaya T, Yasuike M, Fujiwara A, Kondo H, Hirono I, Fukuda Y, Nakayasu C. Complete Genome Sequence of Ichthyobacterium seriolicida JBKA-6(T), Isolated from Yellowtail (Seriola quinqueradiata) Affected by Bacterial Hemolytic Jaundice. Genome Announc. 2017. 5(6). e01574-16.
  • Matsuyama T, Fukuda Y, Sakai T, Tanimoto N, Nakanishi M, Nakamura Y, Takano T, Nakayasu C. Clonal structure in Ichthyobacterium seriolicida, the causative agent of bacterial haemolytic jaundice in yellowtail, Seriola quinqueradiata, inferred from molecular epidemiological analysis. J Fish Dis. 2017. 40(8):1065-1075
  • Viral haemorrhagic septicaemia virus remains viable for several days but at low levels in the water flea Moina macrocopa (Takafumi Ito, Niels Jørgen Olesen, 2017, Diseases of Aquatic Organisms, 127, 11-18)
  • Importation of CyHV-2 infected goldfish into the Netherlands (Takafumi Ito, Jun Kurita, Olga M. Haenen, 2017, Diseases of Aquatic Organisms, 126, 51-62)
  • Takano T, Nawata A, Sakai T, Matsuyama T, Ito T, Kurita J, Terashima S, Yasuike M, Nakamura Y, Fujiwara A, Kumagai A, Nakayasu C. Full-Genome Sequencing and Confirmation of the Causative Agent of Erythrocytic Inclusion Body Syndrome in Coho Salmon Identifies a New Type of Piscine Orthoreovirus. PLoS One. 2016. 11(10):e0165424.
  • 飯田貴次・坂井貴光・高野倫一. エドワジエラ症. 魚病研究. 2016. 51(3): 87-91.
  • Novel viral infections threatening Cyprinid fish (O. Haenen, K. Way, B. Gorgoglione, Takafumi Ito, R. Paley, L. Bigarré and T. Waltzek) 2016, Bulletin of the European Association of Fish Pathologists, 36/1, 11-23)
  • Virulence of viral haemorrhagic septicaemia virus (VHSV) genotype III in rainbow trout (Takafumi Ito, Jun Kurita, Koh-ichiro Mori, Niels Jørgen Olesen, 2016, Veterinary Research, 47, 4)
  • Olga Haenen, Keith Way, Bartolomeo Gorgoglione, Takafumi Ito, Richard Paley, Laurent Bigarré, Thomas Waltzek (2016) Novel viral infections threatening Cyprinid fish. Bulletin of the European Association of Fish Pathologists 36; 11-23
  • Takafumi Ito, Jun Kurita, Koh-ichiro Mori, Niels Jørgen Olesen (2016) Virulence of viral haemorrhagic septicaemia virus (VHSV) genotype III in rainbow trout. Veterinary Research 47; 4
  • Takafumi Ito, Norihisa Oseko, Mitsuru Ototake (2015) Virulence of infectious salmon anemia virus (ISAV) in six Japanese fish species by intraperitoneal injection. Fish Pathology 50; 115-118
  • Takafumi Ito, Norihisa Oseko, Mitsuru Ototake (2015) Susceptibility of Amago trout, Oncorhynchus masou macrostomus (Günther) to an isolate of infectious salmon anaemia virus (ISAV). Journal of Fish Disease 38; 237-240
  • Takafumi Ito, Yukio Maeno (2015) Effect of booster shot and investigation of vaccination efficacy period against herpesviral haematopoietic necrosis (HVHN) in goldfish Carassius auratus. Veterinary Microbiology 175; 139-144
  • Takafumi Ito, Yukio Maeno (2014) Effects of experimentally induced infections of goldfish Carassius auratus with cyprinid herpesvirus 2 (CyHV-2) at various water temperatures. Diseases of Aquatic Organisms 110; 193-200
  • Takafumi Ito, Jun Kurita, Kei Yuasa (2014) Differences in the susceptibility of Japanese indigenous and domesticated Eurasian common carp (Cyprinus carpio), identified by mitochondrial DNA typing, to cyprinid herpesvirus 3 (CyHV-3). Veterinary Microbiology 171; 31-40
  • Takafumi Ito, Yukio Maeno (2014) Susceptibility of Japanese Cyprininae fish species to cyprinid herpesvirus 2 (CyHV-2). Veterinary Microbiology 169; 128-134
  • Goshi Kato, Takamitsu Sakai, Kyuma Suzuki, Natsumi Sano, Tomokazu Takano, Tomomasa Matsuyama, Chihaya Nakayasu (2014) Protective efficacies and immune responses induced by recombinant HCD, atpD and gdhA against bacterial cold-water disease in ayu (Plecoglossus altivelis). Fish and Shellfish Immunology 39; 396-400
  • Takafumi Ito, Mitsuru Ototake (2013) Vaccination against cyprinid herpesvirus 2 (CyHV-2) infection in goldfish Carassius auratus. Bulletin of the European Association of Fish Pathologists 33; 158-164
  • Takafumi Ito, Niels Jørgen Olesen (2013) Susceptibility of various fresh water fish species in Japan to an isolate of viral haemorrhagic septicaemia virus (VHSV) genotype IVb. Diseases of Aquatic Organisms 107; 1-8
  • Takafumi Ito, Jun Kurita, Akiyuki Ozaki, Motohiko Sano, Hideo Fukuda, Mitsuru Ototake (2013) Growth of Cyprinid herpesvirus 2 (CyHV-2) in cell culture and experimental infection of goldfish Carassius auratus. Diseases of Aquatic Organisms 105; 193-202
  • Takafumi Ito, Yasutoshi Yoshiura, Takashi Kamaishi, Kazunori Yoshida, Kazuhiro Nakajima (2013) Prevalence of red sea bream iridovirus (RSIV) among organs of Japanese amberjack (Seriola quinqueradiata) exposed to cultured RSIV. Journal of General Virology 94; 2094-2101
  • Goshi Kato, Takamitsu Sakai, Kyuma Suzuki, Kenichi Yamaguchi, Tomokazu Takano, Tomomasa Matsuyama, Chihaya Nakayasu (2013) Antigenic proteins of Flavobacterium psychrophilum recognized by ayu Plecoglossus altivelis antisera. Diseases of Aquatic Organisms 108; 103-112
  • Takafumi Ito, Jun Kurita, Motohiko Sano, Helle Frank Skall, Niels Lorenzen, Katja Einer-Jensen, Niels Jørgen Olesen (2012) Typing of viral haemorrhagic septicaemia virus by monoclonal antibodies. Journal of General Virology 93; 2546-2557
  • Takamitsu Sakai, Chihaya Nakayasu, Takafumi Ito, Satoshi Miwa, Norihisa Oseko, Takaji Iida (2012) Virulence of Yersinia ruckeri for four indigenous fish species in Japan. Fish Pathology 47; 74-79

刊行図書等

  • 飯田貴次・坂井貴光・高野倫一 (2019) 養殖技術講座 －日本魚病学会 魚類重要疾病対策－ エドワジエラ症 ～ウナギ、ヒラメ、マダイ、アユなど～, 「月刊 養殖ビジネス」, 緑書房, 56(6), pp. 53-57.
  • 坂井貴光 (2015) サケ科魚類のレッドマウス病, 「アクアネット」, 湊文社, 東京, 6, pp. 38-40.